cell lines hek 293t atcc cat Search Results


94
ATCC cell lines hek 293t cells american type culture collection
Cell Lines Hek 293t Cells American Type Culture Collection, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TaKaRa lenti x 293 t cells
Lenti X 293 T Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Biolabs Inc hek 293 cells cat no. aav-100
Hek 293 Cells Cat No. Aav 100, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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European Collection of Authenticated Cell Cultures 293t cells
293t Cells, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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DSMZ acc 635
Acc 635, supplied by DSMZ, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ImmunoGen Inc hek 293t/17 cells
Hek 293t/17 Cells, supplied by ImmunoGen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Millipore genejuice
(A) <t>293T</t> cells were assessed for the surface presentation of known CHIKV attachment factors (TIM-1, MXRA8, or L-SIGN) via flow cytometry. 293T cells were transfected with either TIM-1, MXRA9, L-SIGN, or GFP 24 hrs prior to the addition of primary antibodies specific against hTIM-1, MXRA8, or L-SIGN. 24 hrs post transfection, 293T, HAP1, and VeroS cells were inoculated with either ( B-D ) mKate-expressing CHIKV strain 181/c25, ( E-G ) recombinant vesicular stomatitis virus containing the Lassa virus glycoprotein (rVSVΔG/LASV), or ( H-J ) rVSV studded with the Ebola virus glycoprotein (rVSVΔG/EBOV) for 1 hr. 12 hrs post infection, cells were assessed for CHIKV infection (mKate + ) and transfection efficiency (GFP + ) via flow cytometry. Relative infection was calculated as the proportion of cells infected with CHIKV (mKate + ) among transfected cells (GFP + ) normalized to infection levels in a GFP only control well. At least three independent replicates were performed with each bar representing the mean and error (±SEM) with an unpaired parametric Student’s T-test with unequal variance (Welch’s correction) was used to determine statistical significance, where * ( p < 0.05), ** ( p < 0.01), *** ( p < 0.001).
Genejuice, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Thermo Fisher flp-in 293 t-rex cells
(A) <t>293T</t> cells were assessed for the surface presentation of known CHIKV attachment factors (TIM-1, MXRA8, or L-SIGN) via flow cytometry. 293T cells were transfected with either TIM-1, MXRA9, L-SIGN, or GFP 24 hrs prior to the addition of primary antibodies specific against hTIM-1, MXRA8, or L-SIGN. 24 hrs post transfection, 293T, HAP1, and VeroS cells were inoculated with either ( B-D ) mKate-expressing CHIKV strain 181/c25, ( E-G ) recombinant vesicular stomatitis virus containing the Lassa virus glycoprotein (rVSVΔG/LASV), or ( H-J ) rVSV studded with the Ebola virus glycoprotein (rVSVΔG/EBOV) for 1 hr. 12 hrs post infection, cells were assessed for CHIKV infection (mKate + ) and transfection efficiency (GFP + ) via flow cytometry. Relative infection was calculated as the proportion of cells infected with CHIKV (mKate + ) among transfected cells (GFP + ) normalized to infection levels in a GFP only control well. At least three independent replicates were performed with each bar representing the mean and error (±SEM) with an unpaired parametric Student’s T-test with unequal variance (Welch’s correction) was used to determine statistical significance, where * ( p < 0.05), ** ( p < 0.01), *** ( p < 0.001).
Flp In 293 T Rex Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
ATCC human embryonic kidney hek 293t cells
(A) <t>293T</t> cells were assessed for the surface presentation of known CHIKV attachment factors (TIM-1, MXRA8, or L-SIGN) via flow cytometry. 293T cells were transfected with either TIM-1, MXRA9, L-SIGN, or GFP 24 hrs prior to the addition of primary antibodies specific against hTIM-1, MXRA8, or L-SIGN. 24 hrs post transfection, 293T, HAP1, and VeroS cells were inoculated with either ( B-D ) mKate-expressing CHIKV strain 181/c25, ( E-G ) recombinant vesicular stomatitis virus containing the Lassa virus glycoprotein (rVSVΔG/LASV), or ( H-J ) rVSV studded with the Ebola virus glycoprotein (rVSVΔG/EBOV) for 1 hr. 12 hrs post infection, cells were assessed for CHIKV infection (mKate + ) and transfection efficiency (GFP + ) via flow cytometry. Relative infection was calculated as the proportion of cells infected with CHIKV (mKate + ) among transfected cells (GFP + ) normalized to infection levels in a GFP only control well. At least three independent replicates were performed with each bar representing the mean and error (±SEM) with an unpaired parametric Student’s T-test with unequal variance (Welch’s correction) was used to determine statistical significance, where * ( p < 0.05), ** ( p < 0.01), *** ( p < 0.001).
Human Embryonic Kidney Hek 293t Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC human embryonic kidney cells
(A) <t>293T</t> cells were assessed for the surface presentation of known CHIKV attachment factors (TIM-1, MXRA8, or L-SIGN) via flow cytometry. 293T cells were transfected with either TIM-1, MXRA9, L-SIGN, or GFP 24 hrs prior to the addition of primary antibodies specific against hTIM-1, MXRA8, or L-SIGN. 24 hrs post transfection, 293T, HAP1, and VeroS cells were inoculated with either ( B-D ) mKate-expressing CHIKV strain 181/c25, ( E-G ) recombinant vesicular stomatitis virus containing the Lassa virus glycoprotein (rVSVΔG/LASV), or ( H-J ) rVSV studded with the Ebola virus glycoprotein (rVSVΔG/EBOV) for 1 hr. 12 hrs post infection, cells were assessed for CHIKV infection (mKate + ) and transfection efficiency (GFP + ) via flow cytometry. Relative infection was calculated as the proportion of cells infected with CHIKV (mKate + ) among transfected cells (GFP + ) normalized to infection levels in a GFP only control well. At least three independent replicates were performed with each bar representing the mean and error (±SEM) with an unpaired parametric Student’s T-test with unequal variance (Welch’s correction) was used to determine statistical significance, where * ( p < 0.05), ** ( p < 0.01), *** ( p < 0.001).
Human Embryonic Kidney Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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ATCC hek 293 cell line
(A) <t>293T</t> cells were assessed for the surface presentation of known CHIKV attachment factors (TIM-1, MXRA8, or L-SIGN) via flow cytometry. 293T cells were transfected with either TIM-1, MXRA9, L-SIGN, or GFP 24 hrs prior to the addition of primary antibodies specific against hTIM-1, MXRA8, or L-SIGN. 24 hrs post transfection, 293T, HAP1, and VeroS cells were inoculated with either ( B-D ) mKate-expressing CHIKV strain 181/c25, ( E-G ) recombinant vesicular stomatitis virus containing the Lassa virus glycoprotein (rVSVΔG/LASV), or ( H-J ) rVSV studded with the Ebola virus glycoprotein (rVSVΔG/EBOV) for 1 hr. 12 hrs post infection, cells were assessed for CHIKV infection (mKate + ) and transfection efficiency (GFP + ) via flow cytometry. Relative infection was calculated as the proportion of cells infected with CHIKV (mKate + ) among transfected cells (GFP + ) normalized to infection levels in a GFP only control well. At least three independent replicates were performed with each bar representing the mean and error (±SEM) with an unpaired parametric Student’s T-test with unequal variance (Welch’s correction) was used to determine statistical significance, where * ( p < 0.05), ** ( p < 0.01), *** ( p < 0.001).
Hek 293 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Beyotime 293t cells
(A) <t>293T</t> cells were assessed for the surface presentation of known CHIKV attachment factors (TIM-1, MXRA8, or L-SIGN) via flow cytometry. 293T cells were transfected with either TIM-1, MXRA9, L-SIGN, or GFP 24 hrs prior to the addition of primary antibodies specific against hTIM-1, MXRA8, or L-SIGN. 24 hrs post transfection, 293T, HAP1, and VeroS cells were inoculated with either ( B-D ) mKate-expressing CHIKV strain 181/c25, ( E-G ) recombinant vesicular stomatitis virus containing the Lassa virus glycoprotein (rVSVΔG/LASV), or ( H-J ) rVSV studded with the Ebola virus glycoprotein (rVSVΔG/EBOV) for 1 hr. 12 hrs post infection, cells were assessed for CHIKV infection (mKate + ) and transfection efficiency (GFP + ) via flow cytometry. Relative infection was calculated as the proportion of cells infected with CHIKV (mKate + ) among transfected cells (GFP + ) normalized to infection levels in a GFP only control well. At least three independent replicates were performed with each bar representing the mean and error (±SEM) with an unpaired parametric Student’s T-test with unequal variance (Welch’s correction) was used to determine statistical significance, where * ( p < 0.05), ** ( p < 0.01), *** ( p < 0.001).
293t Cells, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) 293T cells were assessed for the surface presentation of known CHIKV attachment factors (TIM-1, MXRA8, or L-SIGN) via flow cytometry. 293T cells were transfected with either TIM-1, MXRA9, L-SIGN, or GFP 24 hrs prior to the addition of primary antibodies specific against hTIM-1, MXRA8, or L-SIGN. 24 hrs post transfection, 293T, HAP1, and VeroS cells were inoculated with either ( B-D ) mKate-expressing CHIKV strain 181/c25, ( E-G ) recombinant vesicular stomatitis virus containing the Lassa virus glycoprotein (rVSVΔG/LASV), or ( H-J ) rVSV studded with the Ebola virus glycoprotein (rVSVΔG/EBOV) for 1 hr. 12 hrs post infection, cells were assessed for CHIKV infection (mKate + ) and transfection efficiency (GFP + ) via flow cytometry. Relative infection was calculated as the proportion of cells infected with CHIKV (mKate + ) among transfected cells (GFP + ) normalized to infection levels in a GFP only control well. At least three independent replicates were performed with each bar representing the mean and error (±SEM) with an unpaired parametric Student’s T-test with unequal variance (Welch’s correction) was used to determine statistical significance, where * ( p < 0.05), ** ( p < 0.01), *** ( p < 0.001).

Journal: bioRxiv

Article Title: Phosphatidylserine within the Viral Membrane Enhances Chikungunya Virus Infectivity in a Cell-type Dependent Manner

doi: 10.1101/2022.01.14.476428

Figure Lengend Snippet: (A) 293T cells were assessed for the surface presentation of known CHIKV attachment factors (TIM-1, MXRA8, or L-SIGN) via flow cytometry. 293T cells were transfected with either TIM-1, MXRA9, L-SIGN, or GFP 24 hrs prior to the addition of primary antibodies specific against hTIM-1, MXRA8, or L-SIGN. 24 hrs post transfection, 293T, HAP1, and VeroS cells were inoculated with either ( B-D ) mKate-expressing CHIKV strain 181/c25, ( E-G ) recombinant vesicular stomatitis virus containing the Lassa virus glycoprotein (rVSVΔG/LASV), or ( H-J ) rVSV studded with the Ebola virus glycoprotein (rVSVΔG/EBOV) for 1 hr. 12 hrs post infection, cells were assessed for CHIKV infection (mKate + ) and transfection efficiency (GFP + ) via flow cytometry. Relative infection was calculated as the proportion of cells infected with CHIKV (mKate + ) among transfected cells (GFP + ) normalized to infection levels in a GFP only control well. At least three independent replicates were performed with each bar representing the mean and error (±SEM) with an unpaired parametric Student’s T-test with unequal variance (Welch’s correction) was used to determine statistical significance, where * ( p < 0.05), ** ( p < 0.01), *** ( p < 0.001).

Article Snippet: All HAP1 cells were transfected with JetOptimus (PolyPlus, cat. 117-07), VeroS, VeroSΔXKR8 and 293T cells with GeneJuice (Sigma-Alrich, cat. 70967), and CDC50A KO lines with Viafect (Promega, cat. E4981) according to manufacturer recommendations.

Techniques: Flow Cytometry, Transfection, Expressing, Recombinant, Infection