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Image Search Results
Journal: bioRxiv
Article Title: Phosphatidylserine within the Viral Membrane Enhances Chikungunya Virus Infectivity in a Cell-type Dependent Manner
doi: 10.1101/2022.01.14.476428
Figure Lengend Snippet: (A) 293T cells were assessed for the surface presentation of known CHIKV attachment factors (TIM-1, MXRA8, or L-SIGN) via flow cytometry. 293T cells were transfected with either TIM-1, MXRA9, L-SIGN, or GFP 24 hrs prior to the addition of primary antibodies specific against hTIM-1, MXRA8, or L-SIGN. 24 hrs post transfection, 293T, HAP1, and VeroS cells were inoculated with either ( B-D ) mKate-expressing CHIKV strain 181/c25, ( E-G ) recombinant vesicular stomatitis virus containing the Lassa virus glycoprotein (rVSVΔG/LASV), or ( H-J ) rVSV studded with the Ebola virus glycoprotein (rVSVΔG/EBOV) for 1 hr. 12 hrs post infection, cells were assessed for CHIKV infection (mKate + ) and transfection efficiency (GFP + ) via flow cytometry. Relative infection was calculated as the proportion of cells infected with CHIKV (mKate + ) among transfected cells (GFP + ) normalized to infection levels in a GFP only control well. At least three independent replicates were performed with each bar representing the mean and error (±SEM) with an unpaired parametric Student’s T-test with unequal variance (Welch’s correction) was used to determine statistical significance, where * ( p < 0.05), ** ( p < 0.01), *** ( p < 0.001).
Article Snippet: All HAP1 cells were transfected with JetOptimus (PolyPlus, cat. 117-07), VeroS, VeroSΔXKR8 and
Techniques: Flow Cytometry, Transfection, Expressing, Recombinant, Infection